Improved determination of D-glucaric acid in urine.

D-GIucaric Acid and Gamma-Glutamyltransferaseas Indicesof Hepatic Enzyme Inductionin Pregnancy

Increased activity of hepatic microsomal enzymes can be evaluatedby measuring D-giucaric acidexcretionin urine and y-glutamyltransferase (EC 2.3.2.2) activity in serum. AsIde from diverse foreign compounds, endogenous steroid hormones have been shown to be normal substrates of the microsomal enzyme system. Because there is an increase In steroid production In pregnancy, we sought to determine w...

[Some considerations on urinary glucaric acid, especially on the relationship between glucaric acid and beta-glucuronidase activity in urine after intravenous administration of xylitol].

Improving D-glucaric acid production from myo-inositol in E. coli by increasing MIOX stability and myo-inositol transport.

D-glucaric acid has been explored for a myriad of potential uses, including biopolymer production and cancer treatment. A biosynthetic route to produce D-glucaric acid from glucose has been constructed in Escherichia coli (Moon et al., 2009b), and analysis of the pathway revealed myo-inositol oxygenase (MIOX) to be the least active enzyme. To increase pathway productivity, we explored protein f...

Urinary D-glucaric acid excretion in the Seveso area, polluted by tetrachloro-dibenzo-p-dioxin (TCDD): five years of experience.

On July 10, 1976, an explosion in a factory in Seveso, Italy, located 30 km north of Milan, producing trichlorophenol caused the release of TCDD-containing compounds in the surrounding area. Since extremely small doses of TCDD have been shown to induce hepatic microsomal enzymes in animals, urinary D-glucaric acid excretion (a measurable index of enzyme induction), has been investigated in Seve...

Improvement of glucaric acid production in E. coli via dynamic control of metabolic fluxes.

D-glucaric acid can be used as a building block for biopolymers as well as in the formulation of detergents and corrosion inhibitors. A biosynthetic route for production in E. coli has been developed (Moon et al., 2009), but previous work with the glucaric acid pathway has indicated that competition with endogenous metabolism may limit carbon flux into the pathway. Our group has recently develo...